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Botany 3% exam weight

Topic 3

Part of the FMGE study roadmap. Botany topic pathol-003 of Botany.

Neoplasia

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Neoplasia — Key Facts for FMGE Core concept: Neoplasia is autonomous, uncontrolled cell proliferation with loss of normal growth control High-yield point: Benign tumors are encapsulated and don’t metastasize; malignant tumors are invasive and can metastasize via blood or lymph ⚡ Exam tip: Grading vs staging and tumor markers are high-yield topics in FMGE


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Neoplasia — FMGE Study Guide

Definitions

Neoplasia: New, uncontrolled growth of cells that exceeds normal tissue architecture and persists after the stimulus is removed

Tumor: Any swelling or mass; technically different from neoplasia but often used interchangeably

Benign tumor: Slow-growing, encapsulated, does not invade or metastasize (suffix: -oma) Malignant tumor: Rapid-growing, invasive, can metastasize (suffix: -carcinoma for epithelial, -sarcoma for mesenchymal)

Nomenclature

Benign Tumors

  • Connective tissue: Lipoma, chondroma, osteoma, fibroma
  • Smooth muscle: Leiomyoma
  • Blood vessels: Hemangioma
  • Glands: Adenoma (benign glandular tumor)

Malignant Tumors

  • Carcinoma: Malignant tumor of epithelial origin (squamous cell carcinoma, adenocarcinoma)
  • Sarcoma: Malignant tumor of mesenchymal origin (osteosarcoma, leiomyosarcoma, fibrosarcoma)
  • Mixed tumors: Contain multiple cell types (e.g., pleomorphic adenoma of salivary gland)
  • Teratoma: Germ cell tumor with multiple tissue types from all three embryologic layers

Special Nomenclature

  • Leukemia: Malignant proliferation of WBCs in blood/bone marrow
  • Lymphoma: Malignant lymphoid tumors
  • Myeloma: Plasma cell neoplasm
  • Seminoma/dysgerminoma: Testicular/ovarian germ cell tumors

Differences Between Benign and Malignant Tumors

FeatureBenignMalignant
Growth rateSlowRapid
CapsulationEncapsulatedNot encapsulated
BordersWell-definedInfiltrative/irregular
MetastasisAbsentPresent
DifferentiationWell-differentiatedPoorly differentiated
MitosisRare, normalFrequent, abnormal
NecrosisRareCommon
RecurrenceRare after removalCommon

Local Invasion and Metastasis

Mechanisms of Spread

Local invasion:

  • Malignant cells lose adhesion molecules (E-cadherin)
  • Secrete proteolytic enzymes (collagenase, MMPs) to degrade basement membrane
  • Direct invasion into surrounding tissue

Lymphatic spread:

  • Carcinomas spread via lymphatics more than sarcomas
  • Sentinel lymph node: First node draining a tumor (important for staging)
  • Can cause lymphangitic carcinomatosis

Hematogenous spread:

  • Invasion of blood vessels (angioinvasion)
  • Sarcomas commonly spread via blood
  • Portal circulation → liver metastases (GI cancers)
  • IVC → lung metastases (renal, testicular cancers)

Patterns of Metastasis

  • Carcinoma: Lungs, liver, brain, bones
  • Sarcoma: Lungs (most common)
  • Prostate cancer: Bones (osteoblastic metastases)
  • Breast cancer: Bones, brain, liver, lungs
  • Colon cancer: Liver (portal drainage)

Seed and soil hypothesis: Certain tumors metastasize to specific organs due to microenvironment compatibility

Grading and Staging

Grading (Tumor Characteristics)

Based on degree of differentiation and mitotic activity:

  • Grade I (G1): Well-differentiated, low mitotic rate
  • Grade II (G2): Moderately differentiated
  • Grade III (G3): Poorly differentiated
  • Grade IV (G4): Anaplastic/undifferentiated

TNM Staging System

  • T: Tumor size and local extent (T1-T4)
  • N: Lymph node involvement (N0-N3)
  • M: Distant metastasis (M0-M1)

Stage grouping:

  • Stage 0: Carcinoma in situ
  • Stage I: Small, localized tumor
  • Stage II: Larger or with minimal spread
  • Stage III: Extensive local/regional spread
  • Stage IV: Distant metastasis

Oncogenes and Tumor Suppressor Genes

Oncogenes (Growth-promoting genes)

  • ras: GTPase signal transducer; mutated in many cancers (30% of all human tumors)
  • myc: Transcription factor; overexpressed in Burkitt lymphoma (t(8;14))
  • bcl-2: Prevents apoptosis; overexpressed in follicular lymphoma t(14;18)
  • Her2/neu (ERBB2): Receptor tyrosine kinase; amplified in breast cancer
  • bcr-abl: Tyrosine kinase; fusion in CML t(9;22) - targeted by imatinib
  • EGFR: Growth factor receptor; mutated in lung adenocarcinoma

Tumor Suppressor Genes (Growth-inhibiting genes)

  • p53: “Guardian of genome” - most frequently mutated gene in human cancers; prevents DNA-damaged cells from replicating
  • Rb (Retinoblastoma): Blocks cell cycle at G1/S checkpoint; both alleles must be lost
  • APC: Adenomatous polyposis coli; loss → familial adenomatous polyposis (FAP) → colon cancer
  • BRCA1/BRCA2: DNA repair; mutations → breast and ovarian cancer
  • WT1: Wilms tumor suppressor
  • NF1/NF2: Neurofibromatosis (nerve sheath tumors)

Carcinogenic Agents

Chemical Carcinogenesis

Initiators (irreversible):

  • Polycyclic aromatic hydrocarbons: Benzopyrene (smoke, charcoal-grilled meat) → lung, skin cancer
  • Aromatic amines: 2-naphthylamine → bladder cancer
  • Nitrosamines: Gastric cancer
  • Alkylating agents: Chemotherapy agents (cyclophosphamide)

Promoters (reversible, require initiator):

  • Phenobarbital (promotes liver tumors)
  • Hormones (estrogen)

Carcinogenesis stages: Initiation (mutations) → Promotion (clonal expansion) → Progression (malignant phenotype)

Viral Oncogenesis

  • HPV (Human Papillomavirus): E6 protein inactivates p53, E7 inactivates Rb → cervical carcinoma
  • HBV, HCV: Chronic hepatitis → hepatocellular carcinoma
  • EBV: Burkitt lymphoma, nasopharyngeal carcinoma, post-transplant lymphoproliferative disorder
  • HTLV-1: Adult T-cell leukemia/lymphoma
  • HHV-8 (Kaposi sarcoma-associated herpesvirus): Kaposi sarcoma

Radiation Carcinogenesis

  • UV radiation: Basal cell carcinoma, squamous cell carcinoma, melanoma (DNA damage, pyrimidine dimers)
  • Ionizing radiation: Leukemia (atomic bomb survivors), thyroid cancer (radioiodine)

Tumor Markers

MarkerAssociated Tumor
AFPHepatocellular carcinoma, non-seminomatous germ cell tumors
CEAColorectal cancer (also raised in pancreatitis, smoking)
CA-125Ovarian cancer
CA-19-9Pancreatic cancer
PSAProstate cancer
β-hCGChoriocarcinoma, testicular germ cell tumors
CalcitoninMedullary thyroid carcinoma
S-100Melanoma
Alkaline phosphataseBone metastases (osteoblastic), liver metastases

Paraneoplastic Syndromes

Symptoms produced by tumors at sites distant from the tumor itself:

  • PTHrP (Parathyroid hormone-related peptide): Hypercalcemia (squamous cell lung cancer, breast cancer)
  • ACTH: Cushing syndrome (small cell lung cancer)
  • ADH: SIADH (small cell lung cancer)
  • EPO: Polycythemia (renal cell carcinoma, cerebellar hemangioma)
  • 5-HT (Serotonin): Carcinoid syndrome (bronchial adenoma, GI carcinoid)
  • Calcitonin: Diarrhea (medullary thyroid carcinoma)

Hallmarks of Cancer (Hanahan and Weinberg)

  1. Self-sufficiency in growth signals
  2. Insensitivity to growth-inhibitory signals
  3. Evasion of apoptosis
  4. Limitless replicative potential (telomerase activation)
  5. Sustained angiogenesis
  6. Ability to invade and metastasize
  7. Reprogramming energy metabolism (Warburg effect)
  8. Evasion of immune destruction

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