Skip to main content
Botany 3% exam weight

Topic 8

Part of the INI CET (AIIMS PG) study roadmap. Botany topic pharma-008 of Botany.

Antimicrobial Agents: General Principles covers antimicrobial agents — general principles for INI CET (AIIMS PG).

Key Definitions:

  • Bacteriostatic: Inhibits bacterial growth (not killing) — tetracyclines, macrolides, sulfonamides
  • Bactericidal: Kills bacteria — penicillins, cephalosporins, aminoglycosides, fluoroquinolones, metronidazole
  • Minimum Inhibitory Concentration (MIC): Lowest drug concentration that prevents visible bacterial growth
  • Minimum Bactericidal Concentration (MBC): Lowest drug concentration that kills 99.9% of bacteria
  • Breakpoint: MIC threshold defining susceptible (S) vs resistant (R)

Classification of Antimicrobials:

1. Cell Wall Synthesis Inhibitors (most are bactericidal):

  • β-lactams: Penicillins, Cephalosporins, Carbapenems, Monobactams
  • Glycopeptides: Vancomycin, Teicoplanin
  • Others: Daptomycin (lipopeptide — disrupts membrane)

2. Protein Synthesis Inhibitors (bacteriostatic — except aminoglycosides):

  • 30S inhibitors: Aminoglycosides (bactericidal), Tetracyclines (bacteriostatic)
  • 50S inhibitors: Macrolides (azithromycin, clarithromycin), Lincosamides (clindamycin), Chloramphenicol, Linezolid (oxazolidinone)

3. Nucleic Acid Synthesis Inhibitors:

  • Folate pathway: Sulfonamides, Trimethoprim (separate steps — synergistic)
  • DNA gyrase/topoisomerase IV: Fluoroquinolones (bactericidal)
  • RNA polymerase: Rifampin (bactericidal — used in TB)
  • DNA: Metronidazole (bactericidal — anaerobes)

4. Cell Membrane Disruptors:

  • Polymyxins (colistin — last resort for gram-negatives)
  • Daptomycin (gram-positive)

5. Anti-TB Agents: Isoniazid, Rifampin, Pyrazinamide, Ethambutol, Streptomycin

6. Anti-Fungal: Polyenes (amphotericin B, nystatin), Azoles, Echinocandins, Flucytosine

Selective Toxicity: The fundamental principle — drug must be more toxic to the pathogen than to the host.

  • Human cells lack cell walls → β-lactams are selectively toxic to bacteria
  • Bacterial ribosomes (70S) differ from human (80S) → aminoglycosides selectively target bacterial protein synthesis
  • Fungal ergosterol vs human cholesterol → polyene antifungals selectively toxic to fungi

Spectrum of Activity:

  • Narrow spectrum: Single organism group (e.g., penicillin G — mostly gram-positive cocci)
  • Broad spectrum: Multiple organism groups (e.g., fluoroquinolones, carbapenems)
  • Superinfection: Overgrowth of resistant organisms following broad-spectrum antibiotic use (e.g., C. difficile colitis from clindamycin, fluoroquinolones)

Combination Therapy Rationale:

  • Broad empiric coverage (unknown pathogen)
  • Synergy (e.g., β-lactam + aminoglycoside for Pseudomonas)
  • Prevent resistance emergence (e.g., TB — multiple drugs prevent resistant strains)
  • Polymicrobial infections (e.g., intra-abdominal infections — multiple organisms)

Exam Tip for INI CET (AIIMS PG): Bacteriostatic drugs (tetracyclines, macrolides, sulfonamides) may antagonize bactericidal drugs (β-lactams, aminoglycosides) — avoid combining in serious infections. Exception: Trimethoprim-sulfamethoxazole (TMP-SMX) is synergistic despite both being bacteriostatic.